Human Neuronal SHSY5Y Cells on PVDF:PTrFE Copolymer Thin Films

Ilaria Tonazzini, Eva Bystrenova, Beatrice Chelli, Pierpaolo Greco, Dago De Leeuw and Fabio Biscarini

Adv. Eng. Mater. 2015, 17(7), 1051-1056.

Abstract:

Here, we investigate the in vitro biocompatibility of polyvinylidenefluoride (PVDF)–polytrifluoroethylene (PTrFE) copolymer thin films using neuronal cells. The aim is to obtain an optimal capping layer material for hybrid bio-organic electronic transducers based on organic electronic devices, which can be applied for bidirectional communication with the central nervous system. PVDF:PTrFE thin films were grown by spin coating and characterized by AFM. Human neuroblastoma SHSY5Y cells were used as neuronal model and their interaction with PVDF:PTrFE films was investigated in term of cell adhesion, proliferation, differentiation, and cytoskeleton organization. We show that PVDF:PTrFE thin films allowed standard SHSY5Y proliferation and neuronal differentiation, even if with reduced short-term cell adhesion and spreading.